Intercellular adhesion molecule-1 (ICAM1) is a ligand for lymphocyte function-associated (LFA) antigens.
Intercellular adhesion molecule-1 (ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for LFA-1, a beta 2 leukocyte integrin.
ICAM1 protein is the major human rhinovirus receptor.
ICAM1 gene is mapped to human chromosome 19.
In humans, lymphocyte adhesion to cells is mediated by the protein heterodimer CD11a/CD18 (Leu-CAMa, LFA-1) and its ligand CD54 (ICAM-1).
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ug
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity:Recognizes human ICAM1.
Isotype:IgG
Calc Applications Abbrev:IHC WB
Calc Crossreactivity:Hu
Immunogen:Recombinant protein corresponding to human ICAM1 expressed in NSO cells. (Position: Q28-E480). Species sequence homology: mouse (55%) and rat (53%)
Shelf Life:1year
Swiss Prot Number:P05362
EU Commodity Code:30021010
商品名:ICAM1 (Antigen Identified by Monoclonal Antibody BB2, BB 2, BB2, CD 54, CD_Antigen, CD54, CD54 Antigen, Cell Surface Glycoprotein P3.58, Human Rhinovirus Receptor, ICAM 1, ICAM-1, ICAM1_HUMAN, Intercellular Adhesion Molecule 1 (CD54), Human Rhinovirus Receptor, Intercellular Adhesion Molecule 1, Major Group Rhinovirus Receptor, MALA 2, MALA2, MyD 10, MyD10, P3.58, Surface Antigen of Activated B Cells, Surface Antigen of Activated B Cells, BB2) 100ug