84-3432-92　Hsp90alpha (EL52, Epididymis Luminal Secretory Protein 52, Heat Shock 86kD, Heat Shock 90kD Protein 1, alpha, Heat Shock 90kD Protein 1, alpha Like 4, Heat Shock 90kD Protein, alpha-like 4, Heat Shock 90kD Protein 1 alpha, Heat Shock Protein 90kD alpha (Cytosolic) Class A Member 1, Heat Shock Protein 90kD alpha (Cytosolic), Class A Member 2, Heat Shock Protein HSP 90-alpha, HSP 86, HSP86, Hsp8 ...　303368

特徴

• Heat shock protein HSP 90-alpha is a protein that in humans is encoded by the HSP90AA1 gene.
• The gene, HSP90AA1, encodes the human stress-inducible 90kD heat shock protein alpha (Hsp90A).
• Complemented by the constitutively expressed paralog Hsp90B which shares over 85% amino acid sequence identity, Hsp90A expression is initiated when a cell experiences proteotoxic stress.
• Once expressed Hsp90A dimers operate as molecular chaperones that bind and fold other proteins into their functional 3-dimensional structures.
• This molecular chaperoning ability of Hsp90A is driven by a cycle of structural rearrangements fueled by ATP hydrolysis.
• Current research on Hsp90A focuses in its role as a drug target due to its interaction with a large number of tumor promoting proteins and its role in cellular stress adaptation.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions
• Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
• Immunocytochemistry: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.