HSP60 is a member of the chaperonin class of protein factors, which include the Escherichia coli groEL protein and the Rubisco subunit-binding protein of chloroplasts.
It acts as a costimulator of human regulatory CD4-positive/CD25 -positive T cells, which inhibit lymphoproliferation and IFNG and TNF secretion by CD4-positive and CD8-positive T cells.
HSP60 enhances Treg activity via TLR2, leading to activation of an intracellular signaling cascade that included p38, as well as inhibition of ERK phosphorylation.
Suppression of target T cells is mediated by both cell-to-cell contact and by secretion of TGFB and IL10, and it leads to downregulation of ERK, NFKB, and TBET expression.
The self-molecule HSP60 can downregulate adaptive immune responses by upregulating Tregs through TLR2 signaling.
Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested
Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.1ng/lane under reducing conditions
Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
Immunocytochemistry: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human Hsp60. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human Hsp60 expressed in E. coli. (Position: A260-Q496). Species sequence homology: mouse and rat (97%)
Shelf Life：1year
Swiss Prot Number：P10809
EU Commodity Code：30021010
商品名：Hsp60 (60kD Chaperonin, 60kD Heat Shock Protein, Mitochondrial, 60kD, Cb863, CH60_HUMAN, Chaperonin 60, Chaperonin, Chaperonin, 60kD, CPN60, GROEL, GroEL, E. coli, Homolog of, Heat Shock 60kD Protein 1 (Chaperonin), Heat Shock 60kD Protein 1 (Chaperonin), Heat Shock 60kD Protein 1, Heat Shock Protein 1 (Chaperonin), Heat Shock Protein 60, Heat Shock Protein 65, HLD4, Hsp 60, HSP 65, HSP-60, HSP60, HSP65, HSPD1, HuCHA60, Mitochondrial Matrix Protein P1, P60 Lymphocyte Protein, Short Heat Shock Protein 60 Hsp60s1, Spastic Paraplegia 13 (Autosomal Dominant), SPG13) 100ug
