Heat shock protein 47, also known as SERPINH1 or HSP47, is a serpin which serves as a human chaperone protein for collagen.
This protein is a member of the serpin superfamily of serine proteinase inhibitors.
Its expression is induced by heat shock.
The protein localizes to the endoplasmic reticulum lumen and binds collagen; thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules.
Autoantibodies to this protein have been found in patients with rheumatoid arthritis.
It has been found that HSP47 monitors the integrity of the triple helix of type I procollagen at the ER/cis-Golgi boundary and, when absent, the rate of transit from the ER to the Golgi is increased and the helical structure is compromised.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions
Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human Hsp47. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Rt
Immunogen：Recombinant protein corresponding to human Hsp47 expressed in E. coli. (Position: D247-L418). Species sequence homology: mouse and rat (97%)
Shelf Life：1year
Swiss Prot Number：P50454
EU Commodity Code：30021010
商品名：Hsp47 (47kD Heat Shock Protein, 47kD Heat Shock Protein Precursor, Arsenic Transactivated Protein 3, Arsenic-transactivated Protein 3, AsTP 3, AsTP3, CBP 1, CBP 2, CBP1, CBP2, Cell Proliferation-inducing Gene 14 Protein, Collagen Binding Protein 1, Collagen Binding Protein 2, Collagen Binding Protein, Collagen-binding Protein 2, Collagen-binding Protein, Colligen, Colligin 1, Colligin 2, Colligin, Colligin-1, Colligin-2, Gp46, Heat Shock Protein 47, Heat-shock Protein 47, Hsp 47, J6, OI10, PIG 14, PIG14, PPROM, Proliferation Inducing Gene 14, Proliferation Inducing Gene 14 Protein, RA A47, RA-A47, Rheumatoid Arthritis Antigen A 47, Rheumatoid Arthritis Antigen A-47, Rheumatoid Arthritis Related Antigen RA A47, Rheumatoid Arthritis-related Antigen RA-A47, Serine (or Cysteine) Proteinase Inhibitor, Clade H (Heat Shock Protein 47), Member 1, (Collagen Binding Protein 1), Serine (or Cysteine) Proteinase Inhibitor, Clade H (Heat Shock Protein 47), Member 2, (Collagen-binding Protein 2), Serine or Cysteine Proteinase Inhibitor Clade H Member 1, Serine or Cysteine Proteinase Inhibitor Clade H Member 2, SERPH_HUMAN, Serpin H1, Serpin Peptidase Inhibitor Clade H Member 1, Serpin Peptidase Inhibitor, Clade H (Heat Shock Protein 47), Member 1, (Collagen Binding Protein 1), Serpin Peptidase Inhibitor, Clade H, Member 1, SERPINH1, SERPINH2) 100ug
