84-3432-84　HLA-C, CT (1C07_HUMAN, Cw-7 alpha Chain, HLA Class I Histocompatibility Antigen, HLA Class I Histocompatibility Antigen, C alpha Chain, HLA Class I Histocompatibility Antigen, Cw-1 alpha Chain, HLA-C Histocompatibility Type, HLA-Cw, HLA-JY3, HLAC, HLC-C, Human Leukocyte Antigen-C alpha Chain, Major Histocompatibility Antigen HLA-C, Major Histocompatibility Complex, Class I, C, MHC Class I Anti ...　303359

特徴

• HLA-C belongs to the HLA class I heavy chain paralogues.
• This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin).
• The heavy chain is anchored in the membrane.
• Class I molecules play a central role in the immune system by presenting peptides derived from endoplasmic reticulum lumen.
• They are expressed in nearly all cells.
• The heavy chain is ~45kD and its gene contains 8 exons.
• Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail.
• Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule.
• Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation.
• Over one hundred HLA-C alleles have been described.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.1ng/lane under reducing conditions
• Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
• Immunocytochemistry: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.