HEXA (hexosaminidase A (alpha polypeptide)) is an enzyme that in humans is encoded by the HEXA gene.
Hexosaminidase A and the cofactor GM2 activator protein catalyze the degradation of the GM2 gangliosides and other molecules containing terminal N-acetyl hexosamines.
The HEXA gene encodes the alpha subunit of hexosaminidase A, a lysosomal enzyme involved in the breakdown of gangliosides.
The HEXA gene is mapped on 15q23.
Even though the alpha and beta subunits of hexosaminidase A can both cleave GalNAc residues, only the alpha subunit is able to hydrolyze GM2 gangliosides.
The alpha subunit contains a key residue, Arg-424, which is essential for binding the N-acetyl-neuramanic residue of GM2 gangliosides.
Chimeric constructs were expressed in HeLa cells and selected constructs were produced in the baculovirus expression system to determine their ability to degrade GM2 ganglioside in the presence of GM2 activator protein.
Their results allowed them to define 2 noncontiguous sequences in the alpha subunit (aa1-191 and aa403-529) which, when substituted into analogous positions in the beta subunit, conferred activity against the sulfated substrate.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human HEXA.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa513-529, QAQPLNVGFCEQEFEQT, from human HEXA at C-terminus, different from the related mouse sequence by 3aa, and from the related rat sequences by 4aa.
Shelf Life：1year
Swiss Prot Number：P06865
EU Commodity Code：30021010
商品名：HEXA, CT (Beta Hexosaminidase Alpha Chain Precursor, Beta Hexosaminidase Subunit Alpha, Beta N Acetylhexosaminidase, Beta N Acetylhexosaminidase Subunit Alpha, Beta-hexosaminidase Subunit Alpha, Beta-N-acetylhexosaminidase Subunit Alpha, Hexa, HEXA_HUMAN, Hexosaminidase A (Alpha Polypeptide), Hexosaminidase A Alpha Polypeptide, Hexosaminidase A, Hexosaminidase Subunit A, N Acetyl Beta Glucosaminidase, N Acetyl Beta Glucosaminidase Subunit Alpha, N-acetyl-beta-glucosaminidase Subunit Alpha, TSD) 100ug
