Hamartin, also known as tuberous sclerosis 1, is a protein that in humans is encoded by the TSC1 gene.
It is mapped to 9q34.13.
This peripheral membrane protein was implicated as a tumor suppressor.
It forms a complex with TSC2 that regulates mTORC1 signaling and may be also involved in vesicular transport and docking.
Hamartin and TSC2 have critical roles in neuronal polarity, and that a common pathway regulates polarization and growth in neurons and cell size in other tissues.
Hamartin is a growth inhibitory protein whose biologic effect is probably dependent on its interaction with tuberin.
It also can affect cell proliferation via deregulation of G1 phase.
Loss or perturbation of Hamartin function leads to loss of adhesion to the cellular matrix and initiates the development of TSC hamartomas.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human Hamartin. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Rt
Immunogen：Recombinant protein corresponding to human Hamartin expressed in E. coli. (Position: D686-Y884). Species sequence homology: mouse (96%) and rat (95%)
