84-3432-61　GLUT4 (Insulin-responsive, Glucose Transporter GLUT 4, Glucose Transporter Type 4, Glucose Transporter Type 4 Insulin Responsive, GLUT 4, GLUT-4, GTR4_HUMAN, Kug, SLC 2A4, SLC2A4, Solute Carrier Family 2 (Facilitated Glucose Transporter) Member 4, Solute Carrier Family 2, Facilitated Glucose Transporter Member 4) 100ug　303335

特徴

• GLUT4, also known as SLC2A4 or solute carrier family 2 (facilitated glucose transporter) member 4, is a protein that in humans is encoded by the GLUT4 gene.
• It is mapped to 17p13.1.
• This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter.
• In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue.
• Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane.
• Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM).
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions
• Immunohistochemistry (frozen): 0.5-1ug/mlImmunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.