84-3432-56　GFRA1 (GDNF Family Receptor Alpha 1, GDNF R, GDNF RA, GDNF Receptor alpha, GDNFR alpha 1, GDNFR alpha, GDNFR, GDNFRA, GDNFRalpha, GFR alpha 1, GFR alpha1, GFR-Alpha-1, GFRA 1, GFRalpha1, Glial Cell Line Derived Neurotrophic Factor Receptor Alpha, Glial Cell Line-derived Neurotrophic Factor Receptor alpha, GPI Linked Anchor Protein, GPI-linked Anchor Protein, PI Linked Cell Surface Accessory Pr ...　303330

特徴

• GDNF family receptor alpha-1 (GFRa1), also known as the GDNF receptor or GFRA1, is a protein that in humans is encoded by the GFRA1 gene.
• It is mapped to chromosome 10q25.3.
• The protein encoded by this gene is a member of the GDNF receptor family.
• GFRA1 is released by neuronal cells, Schwann cells, and injured sciatic nerve.
• It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor.
• This gene is also a candidate gene for Hirschsprung disease.
• Soluble GFRA1 mediates robust recruitment of RET to lipid rafts via a mechanism requiring the RET tyrosine kinase.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.2ng/lane under reducing conditions
• Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.