84-3432-55　GFAP (Astrocyte, Glial Fibrillary Acidic Protein, Intermediate Filament Protein) 100ug　303329

特徴

• Glial fibrillary acidic protein (GFAP) is a protein that is encoded by the GFAP gene in humans.
• It is an intermediate filament (IF) protein that is expressed by numerous cell types of the central nervous system (CNS) including astrocytes, and ependymal cells.
• It is mapped to 17q21.31.
• GFAP is closely related to its non-epithelial family members, vimentin, desmin, and peripherin, which are all involved in the structure and function of the cell’s cytoskeleton.
• GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of cells.
• This gene has been shown to play a role in mitosis by adjusting the filament network present in the cell.
• GFAP is necessary for many critical roles in the CNS.
• GFAP also plays a role in astrocyte-neuron interactions as well as cell-cell communication.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions
• Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.