Glucose-6-phosphate dehydrogenase, also known as G6PD or G6PDH, is an enzyme that in humans is encoded by the G6PD gene.
It is mapped to Xq28.
G6PD plays a key role in the production of ribose 5-phosphate and the generation of NADPH in the hexose monophosphate pathway.
Because this pathway is the only NADPH-generation process in mature red cells, which lack the citric adid cycle, a genetic deficiency of G6PD is often associated with adverse physiologic effects.
It has been found that aldosterone decreased G6PD expression and activity, resulting in increased oxidant stress and decreased nitric oxide levels, similar to what is observed in G6PD-deficient endothelial cells.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human G6PD.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human G6PD expressed in E. coli. (Position: E315-L515). Species sequence homology: mouse (95%) and rat (96%)
