84-3432-44　FRZB (SFRP3, FIZ, FRE, Frezzled, Fritz, Frizzled-related Protein 1, FRP, FrzB 1, FRZB1, Secreted Frizzled Related Protein 3 [Precursor], Secreted Frizzled Related Sequence Protein 3, SFRP) 100ug　303315

特徴

• FRZB is also known as FRE or OS1.
• The protein encoded by this gene is a secreted protein that is involved in the regulation of bone development.
• Defects in this gene are a cause of female-specific osteoarthritis (OA) susceptibility.
• FRZB is a Wnt-binding protein especially important in embryonic development.
• It is a competitor for the cell-surface G-protein receptor Frizzled.
• FRZB is localized in the extracellular plasma membrane.
• Unlike frizzled, frzb lacks the 7 transmembrane domains normally found in G-protein-coupled receptors.
• It is still considered a homolog of frizzled because it contains a Cysteine Rich Domain (CRD), and because of its intracellular C-terminus which is crucial for signaling.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:ELISA: 0.1-0.5ug/mlWestern Blot: 0.1-0.5ug/ml, the detection limit is ~0.1ng/lane under reducing conditions
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.