Forkhead box protein O1, also known as FKHR or FOXO1A, is a protein that in humans is encoded by the FOXO1 gene.
It is mapped to 13q14.11.
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain.
FOXO1 plays important roles in regulation of gluconeogenesis and glycogenolysis by insulin signaling, and is also central to the decision for a preadipocyte to commit to adipogenesis.
This gene is primarily regulated through phosphorylation on multiple residues.
Its transcriptional activity is dependent on its phosphorylation state.
FOXO1 may play a role in myogenic growth and differentiation.
Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human FOXO1A. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Rt
Immunogen：Recombinant protein corresponding to human FOXO1A expressed in E. coli. (Position: N456-G655). Species sequence homology: mouse and rat (93%)
