84-3432-33　Estrogen Receptor (Atherosclerosis, Susceptibility to, Included, ER Alpha, ER, ER Beta, ER-alpha, ER[a], ER[b], ERA, ERalpha, Erb, Erb2, ERbeta, ESR 1, ESR, ESR Beta, ESR1, ESR1_HUMAN, ESR2, ESRA, ESRB, Estr, Estra, Estradiol Receptor alpha, Estradiol Receptor, Estradiol Receptor beta, ESTRB, Estrogen Receptor 1 (alpha), Estrogen Receptor 1, Estrogen Receptor 2 (ER beta), Estrogen Receptor 2, ...　303301

特徴

• Estrogen receptor alpha (ER-a), also known as NR3A1, is one of two main types of estrogen receptor, a nuclear receptor that is activated by the sex hormone estrogen.
• Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.
• In humans, ER-a is encoded by the gene ESR1 (Estrogen Receptor 1).
• It is mapped to 6q25.1.
• This gene is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription.
• The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2.
• Estrogen and its receptors are essential for sexual development and reproductive function, it also play a role in other tissues such as bone.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.2ng/lane under reducing conditions
• Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.