This gene encodes 3-hydroxyacyl-CoA dehydrogenase type II, a member of the short-chain dehydrogenase/reductase superfamily.
The gene product is a mitochondrial protein that catalyzes the oxidation of a wide variety of fatty acids and steroids, and is a subunit of mitochondrial ribonuclease P, which is involved in tRNA maturation.
The protein has been implicated in the development of Alzheimer disease, and mutations in the gene are the cause of 17beta-hydroxysteroid dehydrogenase type 10 (HSD10) deficiency.
Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.1ng/lane under reducing conditions
Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human ERAB. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding to human ERAB expressed in E. coli. (Position: E48-P261). Species sequence homology: mouse (87%) and rat (88%)
Shelf Life：1year
Swiss Prot Number：Q99714
EU Commodity Code：30021010
商品名：ERAB (17 beta Hydroxysteroid Dehydrogenase 10, 17 beta Hydroxysteroid Dehydrogenase Type 10, 17-beta-HSD 10, 17-beta-hydroxysteroid Dehydrogenase 10, 17b HSD10, 3 Hydroxy 2 Methylbutyryl CoA Dehydrogenase, 3 Hydroxyacyl CoA Dehydrogenase Type 2, 3 Hydroxyacyl CoA Dehydrogenase Type II, 3-hydroxy-2-methylbutyryl-CoA Dehydrogenase, 3-hydroxyacyl-CoA Dehydrogenase type II antibody|3-hydroxyacyl-CoA Dehydrogenase Type-2, AB Binding Alcohol Dehydrogenase, ABAD, Ads9, Amyloid beta Binding Polypeptide, Amyloid beta Peptide Binding Alcohol Dehydrogenase, Amyloid beta Peptide Binding Protein, CAMR, Endoplasmic Reticulum Amyloid Binding Protein, Endoplasmic Reticulum Associated Amyloid beta Peptide Binding Protein, Endoplasmic Reticulum-associated Amyloid beta-peptide-binding Protein, ER Associated Amyloid beta-binding Protein, HADH 2, HADH2, HCD 2, HCD2, HCD2_HUMAN, Hsd17b10, Hydroxyacyl CoA Dehydrogenase Type II, Hydroxyacyl Coenzyme A Dehydrogenase Type II, Hydroxysteroid (17 beta) Dehydrogenase 10, Mental Retardation X Linked Syndromic 11, MHBD, Mitochondrial L3 Hydroxyacyl CoA Dehydrogenase, Mitochondrial Ribonuclease P Protein 2, Mitochondrial RNase P Protein 2, MRPP2, MRX17, SCHAD, SDR5C1, Short Chain Dehydrogenase/Reductase Family 5C Member 1, Short Chain L 3 Hydroxyacyl CoA Dehydrogenase Type 2, Short Chain Type Dehydrogenase/Reductase XH98G2, Short-chain Type Dehydrogenase/Reductase XH98G2, Type 10 17b HSD, Type 10 17beta Hydroxysteroid Dehydrogenase, Type II HADH, XH98G2) 100ug
