Eotaxin-3 is a small cytokine belonging to the CC chemokine family that is also called CCL26, Chemokine (C-C motif) ligand 26.
Chemokines play pivotal roles in health and disease by controlling migration and tissue localization of specific types of cells expressing their cognate receptors.
Eotaxin-3 is chemotactic for eosinophils and basophils and elicits its effects by binding to the cell surface chemokine receptor CCR3.
It is expressed by several tissues including heart, lung and ovary, and in endothelial cells that have been stimulated with the cytokine interleukin 4.
This gene for chemokine is located on human chromosome 7.
Eotaxin-3 induced migration of eosinophils and basophils only at a 10-fold higher concentration than eotaxin.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions
Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human Eotaxin 3.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human Eotaxin 3 expressed in E. coli. (Position: T24-L94).
Shelf Life：1year
Swiss Prot Number：Q9Y258
EU Commodity Code：30021010
商品名：Eotaxin 3 (C-C Motif Chemokine 26, CC Chemokine IMAC, CCL 26, CCL26, Chemokine (C C Motif) Ligand 26, Chemokine N1, Eotaxin-3, IMAC, Macrophage Inflammatory Protein 4-alpha, MIP 4a, MIP 4alpha, MIP-4-alpha, MIP4a, MIP4alpha, SCYA 26, SCYA26, Small Inducible Cytokine A26, Small Inducible Cytokine Subfamily A (Cys Cys) Member 26, Small Inducible Cytokine Subfamily A Member 26, Small-inducible Cytokine A26, Thymic Stroma Chemokine 1, Thymic Stroma Chemokine-1, TSC 1, TSC-1, TSC1) 100ug
