The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands.
It is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4).
EGFR exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor alpha (TGFalpha).
EGFR and its ligands are cell signaling molecules involved in diverse cellular functions, including cell proliferation, differentiation, motility, and survival, and in tissue development.
Mutations that lead to EGFR overexpression (known as upregulation) or overactivity have been associated with a number of cancers, including lung cancer and glioblastoma multiforme.
In this latter case a more or less specific mutation of EGFR, called EGFRvIII is often observed.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human EGFR. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Rt
Immunogen：Recombinant protein corresponding to human EGFR expressed in E. coli. (Position: L25-K346). Species sequence homology: mouse (89%)
Shelf Life：1year
Swiss Prot Number：P00533
EU Commodity Code：30021010
商品名：EGFR (Avian Erythroblastic Leukemia Viral (V Erb b) Oncogene Homolog, Avian Erythroblastic Leukemia Viral (Verbb) Oncogene Homolog, Cell Growth Inhibiting Protein 40, Cell Proliferation Inducing Protein 61, EGF R, EGFR_HUMAN, Epidermal Growth Factor Receptor (Avian Erythroblastic Leukemia Viral (V erb b) Oncogene Homolog), Epidermal Growth Factor Receptor (Erythroblastic Leukemia Viral (V erb b) Oncogene Homolog Avian), Epidermal Growth Factor Receptor, Erbb 1, Erbb, Erbb1, ERBB1, Errp, HER1, mENA, Oncogene ERBB, PIG61, Proto-oncogene C-ErbB-1, Receptor Tyrosine Protein Kinase ErbB 1, Receptor Tyrosine Protein Kinase ErbB1, Receptor Tyrosine-protein Kinase ErbB-1, Urogastrone, Wa2, Wa5) 100ug
