84-3432-23　E-Cadherin (E Cadherin, CDH1, Cadherin-1, ECAD, UVO) 100ug　303290

特徴

• CDH1 (Cadherin 1), also known as ECAD or UVO, is a protein that in humans is encoded by the CDH1 gene.
• Cadherin-1 is a classical member of the cadherin superfamily.
• By Southern analysis of DNA from a panel of mouse-human somatic cell hybrids, Mansouri et al.
• (1987, 1988) assigned the UVO gene to 16q (16p11-qter).
• Frebourg et al.
• (2006) found that in human embryos CDH1 is highly expressed at 4 and 5 weeks in the frontonasal prominence and at 6 weeks in the lateral and medial nasal prominences, and is therefore expressed during critical stages of lip and palate development.
• CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells.
• Has a potent invasive suppressor role.
• It is a ligand for integrin alpha-E/beta-7.
• Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 0.1-0.5ug/mlWestern Blot: 0.1-0.5ug/mlImmunohistochemistry (frozen): 0.5-1ug/mlImmunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.