84-3432-16　Daxx (Death Domain-associated Protein 6, DAP6, BING2, Death-domain Associated Protein) 100ug　303280

特徴

• DAXX (death-domain associated protein) also known as DAP6 (Death-associated protein 6) or BING2, was first discovered through its cytoplasmic interaction with the classical death receptor Fas.
• Human DAXX encodes a 740aa polypeptide containing a nuclear localization signal.
• Functional analyses by Yang et al.
• (1997) demonstrated that Daxx binds to the Fas death domain and enhances Fas-mediated apoptosis.
• The authors suggested that DAXX and FADD define 2 distinct apoptotic pathways downstream of Fas.
• The DAXX gene is mapped to human chromosome 6p21.3 by somatic cell hybrid panels and fluorescence in situ hybridization, a region containing the HLA and putative autoimmune disease genes.
• MSP58 overexpression relieved DAXX-mediated transcriptional repression.
• Immunoprecipitation and Western blot analysis with DAXX mutants showed that the N terminus of DAXX interacts with the C terminus of DMAP.
• Transient expression of DAXX or DMAP1 caused repression of glucocorticoid receptor-mediated transcription.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
• Immunocytochemistry: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.