Cystatin A, also called Stefin A, is a protein that in humans is encoded by the CSTA gene.
Cystatin A is a cysteine proteinase inhibitor that belongs to family 1 of the cystatin superfamily.
This gene was assigned to human chromosome 3q21.1.
This is an intracellular thiol proteinase inhibitor.
Cystatin A plays an important role in desmosome-mediated cell-cell adhesion in the lower levels of the epidermis.
Stefins have been proposed as prognostic and diagnostic tools for cancer.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human Cystatin A.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human Cystatin A expressed in E. coli. (Position: E8-F98). Species sequence homology: mouse (62%) and rat (58%)
