84-3432-08　CYP1A2 (Cytochrome P450 1A2, Cytochrome P450, Subfamily I, Polypeptide 2) 100ug　303272

特徴

• CYP1A2 (Cytochrome P450, Subfamily I, Polypeptide 2) is a member of the cytochrome P450 mixed-function oxidase system and is involved in the metabolism of xenobiotics in the body.
• CYP1A2 is a member of the cytochrome P450 superfamily of enzymes.
• In humans, the CYP1A2 enzyme is encoded by the CYP1A2 gene.
• CYP1A2 localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke.
• The CYP1A2 gene encodes a P450 enzyme involved in O-deethylation of phenacetin.
• Ikeya et al.
• (1989) found that the human CYP1A2 gene spans almost 7.8kb and contains 7 exons.
• The CYP1A2 gene is mapped on 15q24.1.
• CYP1A2 accounts for nearly 15% of the cytochrome P450 in the human liver.
• CYP1A2 displays higher activity in men than in women, and is inhibited by oral contraceptives.
• Inducers of CYP1A2 include cruciferous vegetables.
• Cigarette smoking has also been shown to increase CYP1A2 activity.
• Expression of CYP1A2 appears to be induced by various dietary constituents.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.