CTBP1, C-terminal-binding protein 1, is a protein that in humans is encoded by the CTBP1 gene.
The CtBP1 protein binds to the C-terminus of adenovirus E1A proteins.
This gene is mapped to 4p16.
This phosphoprotein is a transcriptional repressor (corepressor) and may play a role during cellular proliferation.
This protein and the product of a second closely related gene, CTBP2, can dimerize.
CtBP1 and CtBP2 preferentially associate with the E1A via a 5aa motif, PLDLS, to repress E1A induced oncogenesis and cellular transformation.
CtBP1 is expressed from embryo to adult, but CtBP2 is mainly expressed during embryogenesis.
During skeletal and T cell development, CtBP1 and CtBP2 associate with the PLDLSL domain of deltaEF1, a cellular zinc finger-homeodomain protein, and thereby enhances deltaEF1-induced transcriptional silencing.
In addition, CtBP complexes with CtIP, a 125kD protein that recognizes distinctly different protein motifs from CtBP.
CtIP binds to the BRCT repeats within the breast cancer gene BRCA1 and enables CtBP to influence BRCA1 activity.
Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development.
Alternative splicing of transcripts from this gene results in multiple transcript variants.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human CTBP1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa409-440, HGLPPVAHPPHAPSPGQTVKPEADRDHASDQL, from human CTBP1 at C-terminus, different from the related mouse sequence by 1aa, and from the related rat sequence by 2aa.
