84-3431-91　CSK (C SRC, C SRC Kinase, C Src Tyrosine Kinase, C-SRC Kinase, C-src Tyrosine Kinase, Csk A, Cytoplasmic Tyrosine Kinase, P60 Src, Protein Tyrosine Kinase CYL, Protein-tyrosine Kinase CYL, Proto Oncogene Tyrosine Protein Kinase, Tyrosine Protein Kinase CSK, Tyrosine Protein Kinase CSK, Tyrosine-protein Kinase CSK) 100ug　303255

特徴

• CSK also known as C-Src kinase, is a non-receptor protein tyrosine kinase protein that in humans is encoded by the CSK gene.
• It is mapped to 15q24.1.
• CSK suppresses activity of the Src family of protein kinases by phosphorylation of Src family members at a conserved C-terminal site in Src.
• Csk's control of the Src family activity is widely thought to be central to regulation of the immune response.
• CSK can downregulate tyrosine kinase activity of the SRC oncoprotein through tyrosine phosphorylation of the SRC carboxy terminus.
• Since cell transformation by SRC oncoproteins is caused by various mechanisms that interfere with this phosphorylation, the CSK gene might function as an antioncogene.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions
• Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.