CREB1, also known as cAMP responsive element binding protein 1, is a protein that in humans is encoded by the CREB1 gene.
It is mapped to 2q33.3.
This protein binds the cAMP response element, a DNA nucleotide sequence present in many viral and cellular promoters.
The binding of CREB1 stimulates transcription.
This protein is a CREB transcription factor that is a member of the leucine zipper family of DNA-binding proteins.
This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome.
The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human CREB. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Rt
Immunogen：Recombinant protein corresponding to human CREB expressed in E. coli. (Position: M1-T177). Species sequence homology: mouse (99%) and rat (98%)
