84-3431-80　Collagen III, CT (COL3A1, CO3A1_HUMAN, COL 3A1, Collagen alpha 1(III) chain, Collagen alpha-1(III) chain, Collagen III alpha 1 chain precursor, Collagen III alpha 1 polypeptide, Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant), Collagen type III alpha 1, Collagen type III alpha antibody, EDS4A, Ehlers Danlos syndrome type IV, autosomal dominant antibody, Fetal coll ...　303243

特徴

• COL3A1, also called EDS4A or Collagen alpha-1(III), is a protein that in humans is encoded by the COL3A1 gene, it is mapped to 2q32.2.
• COL3A1 chain is a fibrillar-forming collagen comprising 3 alpha-1(III) chains and is expressed in early embryos and throughout embryogenesis.
• In adult, COL3A1 is a major component of the extracellular matrix in a variety of internal organs and skin.
• COL3A1 is also a fibrous scleroprotein in bone, cartilage, dentin, tendon, bone marrow stroma and other connective tissue.
• It is involved in regulation of cortical development, and it Is the major ligand of GPR56 in the developing brain.
• COL3A1 binding to GPR56 can inhibit neuronal migration and activate the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/ml, detection limit is ~0.25ng/lane under reducing conditions
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile dH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.