84-3431-78　CNTF (Ciliary Neurotrophic Factor, HCNTF) 100ug　303241

特徴

• Ciliary neurotrophic factor is a protein that in humans is encoded by the CNTF gene.
• The protein encoded by this gene is a polypeptide hormone and nerve growth factor whose actions have mainly been studied in the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neural populations including astrocytes.
• It is mapped to 11q12.1.
• In addition to neurotrophic effects on parasympathetic neurons, CNTF was shown to have activities on sympathetic and sensory neurons.
• CNTF appears not to be involved in motor neuron survival during development.
• Human CNTF has a trophic influence on degenerating striatal neurons as well as on critical nonstriatal regions.
• CNTF plays a role in a cardiac signal transduction pathway that regulates obesity-related left ventricular hypertrophy.
• Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 0.1-0.5ug/mlWestern Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions
• Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.