84-3431-64　Cdc6 (Cell Division Cycle 6, CDC6, CDC18L, Cell Division Control Protein 6 Homolog) 100ug　303224

特徴

• Cell division control ptotein 6 homolog, also called CDC18L or CDC6, is a protein that in humans is encoded by the CDC6 gene.
• By analysis of somatic cell hybrids and by fluorescence in situ hybridization, this gene is mapped to 17q21.2.
• The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication.
• And this protein functions as a regulator at the early steps of DNA replication.
• It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase.
• The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks.
• Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins.
• This gene is involved in the initiation of DNA replication.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.