84-3431-46　CD20 (APY, ATOPY, B Lymphocyte Antigen CD20, B Lymphocyte Cell Surface Antigen B1, B-lymphocyte Antigen CD20, B-lymphocyte Cell-surface Antigen B1, B-lymphocyte Surface Antigen B1, B1, Bp 35, Bp35, CD 20, CD20 Antigen, CD20 Receptor, CD20_HUMAN, Fc Epsilon Receptor I Beta Chain, Fc Fragment of IgE High Affinity I Receptor for Beta Polypeptide, FCER1B, FCERI, High Affinity Immunoglobulin Epsilo ...　303203

特徴

• CD20, also known as MS4A1, is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R+, CD117+) and progressively increasing in concentration until maturity.
• It is mapped to 11q12.2.
• This gene encodes a member of the membrane-spanning 4A gene family.
• The function of CD20 is to enable optimal B-cell immune response, specifically against T-independent antigens.
• It is suspected that CD20 acts as a calcium channel in the cell membrane.
• This protein may be involved in the regulation of B-cell activation and proliferation.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions
• Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.