ITGAM (Integrin Alpha-M), also called CD11B or Mo1 alpha subunit (MO1A), is one protein subunit that forms the heterodimeric integrin alpha-M beta-2 (alpha M beta2) molecule.
A major surface antigen family on human leukocytes includes complement receptor type 3 (CR3A; also called ITGAM, Mac1, or Mo1), lymphocyte function-associated (LFA) antigen type 1 (ITGAL), and p150, 95 (ITGAX).
By in situ hybridization, Corbi et al.
(1988) demonstrated that the genes encoding the alpha subunits of LFA1 (ITGAL), Mac1, and p150, 95 (ITGAX), all of which are involved in leukocyte adhesion, constitute a cluster on 16p13.1-p11.
Callen et al.
(1991) narrowed the assignment to 16p11.2.
Inflammation plays an essential role in the initiation and progression of atherosclerosis.
Simon et al.
(2000) presented evidence that it also has a role in vascular repair after mechanical arterial injury (i.e., percutaneous transluminal coronary angioplasty, or PTCA).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~1ng/lane under reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human CD11b. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa177-192, MEQLKKSKTLFSLMQY, from human CD11b at N-terminus, different from the related mouse sequence by 1aa, and from the related rat sequence by 3aa.
Shelf Life：1year
Swiss Prot Number：P11215
EU Commodity Code：30021010
商品名：CD11b, NT (Antigen CD11b p170, CD11 Antigen Like Family Member B, CD11 Antigen-like Family Member B, CD11b Antigen, CD11b/CD18, CD49d, Cell Surface Glycoprotein MAC 1 alpha Subunit, Cell Surface Glycoprotein MAC-1 Subunit alpha, Cell Surface Glycoprotein MAC1 Alpha Subunit, Complement Component 3 Receptor 3 Subunit, Complement Component Receptor 3 Alpha, Complement Receptor Type 3, alpha Subunit, CR 3 alpha Chain, CR-3 alpha Chain, CR3 Alpha Chain, CR3, CR3A, Integrin Alpha M, Integrin alpha M Chain, Integrin alpha M Complement Component 3 Receptor 3 Subunit, Integrin alpha-M, Integrin beta 2 alpha Subunit, ITAM_HUMAN, ITGAM, Leukocyte Adhesion Receptor MO1, Ly-40, MAC 1, Mac-1a, MAC1, Mac1, alpha Subunit, MAC1A, Macrophage Antigen alpha Polypeptide, Mo1, alpha Subunit, MO1A, Neutrophil Adherence Receptor alpha M Subunit, Neutrophil Adherence Receptor, SLEB6) 100ug
