Integrin alpha M (ITGAM) is one protein subunit that forms the heterodimeric integrin alpha-M beta-2 (aMb2) molecule, also known as macrophage-1 antigen (Mac-1) or complement receptor 3 (CR3).
It is mapped to 16p11.2.
ITGAM has a role in vascular repair after mechanical arterial injury.
It is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles.
ITGAM probably recognizes the R-G-D peptide in C3b, and it is also a receptor for fibrinogen, factor X and ICAM1.
Applications:Suitable for use in Western Blot, Flow Cytometry, Immunocytochemistry and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
Immunohistochemistry (frozen): 0.5-1ug/mlImmunocytochemistry: 0.5-1ug/mlFlow Cytometry: 1-3ug/1x10e6 cellsOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human CD11b. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：FC IC IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human CD11b at position F17-T382, expressed in E. coli. Species sequence homology: mouse (79%) and rat (61%)
Shelf Life：1year
Swiss Prot Number：P11215
EU Commodity Code：30021010
商品名：CD11b (Integrin alpha M, ITGAM, Antigen CD11b p170, CD11 Antigen Like Family Member B, CD11 Antigen-like Family Member B, CD11b Antigen, CD11b/CD18, CD49d, Cell Surface Glycoprotein MAC 1 alpha Subunit, Cell Surface Glycoprotein MAC-1 Subunit alpha, Cell Surface Glycoprotein MAC1 Alpha Subunit, Complement Component 3 Receptor 3 Subunit, Complement Component Receptor 3 Alpha, Complement Receptor Type 3, alpha Subunit, CR 3 alpha Chain, CR-3 alpha Chain, CR3 Alpha Chain, CR3, CR3A, Integrin Alpha M, Integrin alpha M Chain, Integrin alpha M Complement Component 3 Receptor 3 Subunit, Integrin alpha-M, Integrin beta 2 alpha Subunit, ITAM_HUMAN, Leukocyte Adhesion Receptor MO1, Ly-40, MAC 1, Mac-1a, MAC1, Mac1, alpha Subunit, MAC1A, Macrophage Antigen alpha Polypeptide, Mo1, alpha Subunit, MO1A, Neutrophil Adherence Receptor alpha M Subunit, Neutrophil Adherence Receptor, SLEB6) 100ug
