Macrophage inflammatory protein-1 alpha (MIP-1 alpha), also called CCL3, LD78.
The cDNA for CCL3 predicts a mature peptide of 69aa with a molecular mass of 7,889 daltons.
CCL3 is a member of a newly identified superfamily of small inducible proteins involved in inflammatory responses, wound healing and tumorigenesis.
CCL3 is a chemokine that has pro-inflammatory and stem cell inhibitory activities in vitro.
It constitutes an important second signal for mast cell degranulation in the conjunctiva in vivo and consequently for acute-phase disease.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.1-0.5ug/mlWestern Blot: 0.1-0.5ug/ml, the detection limit is ~1ng/lane under reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 0.9mg sodium chloride, 0.2mg Na2HPO4, 0.05mg sodium azide. Reconstitute with 200ul sterile dH2O to 0.5mg/ml.
Specificity：Recognizes rat CCL3.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to rat CCL3 expressed in E. coli. (Position: A24-A92). Species sequence homology: human (74%) and mouse (91%)
Shelf Life：1year
Swiss Prot Number：P50229
EU Commodity Code：30021010
商品名：CCL3 (C C Motif Chemokine 3, CCL 3, CCL3_HUMAN, Chemokine C C Motif Ligand 3, Chemokine CC Motif Ligand 3, G0/G1 Switch Regulatory Protein 19 1, G0/G1 Switch Regulatory Protein 19-1, G0S19 1, G0S19 1 Protein, Heparin Binding Chemotaxis Protein, L2G25B, LD78 alpha, LD78-alpha(4-69), LD78ALPHA, Macrophage Inflammatory Protein 1 alpha, Macrophage Inflammatory Protein 1-alpha, MIP 1 alpha, MIP 1A, MIP-1-alpha, MIP-1-alpha(4-69), MIP1A, PAT 464.1, SCYA 3, SCYA3, SIS alpha, SIS beta, SIS-beta, Small Inducible Cytokine A3, Small-inducible Cytokine A3, Tonsillar Lymphocyte LD78 alpha Protein) 100ug
