84-3431-37　CBS (Beta Thionase, Beta-thionase, Cbs, Cbs Cystathionine beta-synthase, CBS_HUMAN, Cystathionine beta Synthase, Cystathionine beta-synthase, EC 4.2.1.22, HIP 4, HIP4, Methylcysteine Synthase, Serine Sulfhydrase) 100ug　303192

特徴

• Cystathionine-beta-synthase, also known as CBS, is an enzyme that in humans is encoded by the CBS gene.
• It is mapped to 21q22.3 and contains 23 exons, ranging in size from 42-299bp.
• CBS catalyzes the first step of the transsulfuration pathway, from homocysteine to cystathionine.
• It uses the cofactor pyridoxal-phosphate (PLP) and can be allosterically regulated by effectors such as the ubiquitous cofactor S-adenosyl-L-methionine (adoMet).
• This enzyme belongs to the family of lyases, to be specific, the hydro-lyases, which cleave carbon-oxygen bonds.
• CBS is a multidomain enzyme composed of an N-terminal enzymatic domain and two CBS domains.
• The CBS gene is the most common locus for mutations associated with homocystinuria.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/ml, detection limit is ~0.25ng/lane under reducing conditions
• Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile dH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.