84-3431-36　CBL (C CBL, Cas Br M (Murine) Ecotropic Retroviral Transforming Sequence, Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence, Casitas B Lineage Lymphoma Proto Oncogene, Casitas B-lineage Lymphoma Proto-oncogene, CBL 2, Cbl, CBL2, E3 Ubiquitin Protein Ligase CBL, E3 Ubiquitin-protein Ligase CBL, Oncogene CBL2, Proto Oncogene C CBL, Proto-oncogene C-CBL, RING Finger Protein 55, RNF55, ...　303191

特徴

• CBL (Cbl proto-oncogene) is also known as C-CBL, RNF55, CBL2 and E3 ubiquitin protein ligase.
• CBL is mapped to chromosome 11q23.3-qter by molecular characterization of the breakpoints in 2 somatic cell hybrids.
• The encoded protein is one of the enzymes required for targeting substrates for degradation by the proteasome.
• This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes (E2) to specific substrates.
• This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation.
• As such it functions as a negative regulator of many signal transduction pathways.
• This gene has been found to be mutated or translocated in many cancers including acute myeloid leukaemia.
• Mutations in this gene are also the cause of Noonan syndrome-like disorder.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.