Cathepsin B is an enzymatic protein belonging to the peptidase or protease families.
In humans, it is coded by the CTSB gene.
And this gene is mapped to chromosome 8p22.
The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor.
It is a member of the peptidase C1 family.
Cathepsin B was once suspected as a candidate protease participating in the conversion of beta-amyloid precursor protein into the amyloid plaques found in Alzheimer's disease patients.
However, this function is now known to be mediated by BACE1 protease.
It is now thought that cathepsin B can degrade beta-amyloid precursor protein into harmless fragments.
Thus, it is conceivable cathepsin B may play a pivotal role in the natural defense against Alzheimer's disease.
Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors.
At least five transcript variants encoding the same protein have been found for this gene.
The standard product used in this kit is recombinant human Cathepsin B with the molecular mass of 37kD.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.5ng/lane under reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human Cathepsin B.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human Cathepsin B expressed in E. coli. (Position: L80-D333). Species sequence homology: mouse (83%) and rat (84%)
