Nucleotide-binding oligomerization domain-containing protein 1, also known as CARD4, is a protein receptor that in humans is encoded by the NOD1 gene.
NOD1 is a member of NOD-like receptor protein family and is a close relative of NOD2.
NOD1 is mapped to 7p14.3.
It recognizes bacterial molecules and stimulates an immune reaction.
NOD1 protein contains a caspase recruitment domain (CARD).
This gene is an intracellular pattern recognition receptor, which is similar in structure to resistant proteins of plants, and mediates innate and acquired immunity by recognizing bacterial molecules containing D-glutamyl-meso-diaminopimelic acid (iE-DAP) moiety.
It has been shown that NOD1 can sense cytosolic microbial products by monitoring the activation state of small Rho GTPases.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.2ng/lane under reducing conditions
Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human CARD4. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Rt
Immunogen：Recombinant protein corresponding to human CARD4 expressed in E. coli. (Position: M1-M160). Species sequence homology: mouse (82%)
Shelf Life：1year
Swiss Prot Number：Q9Y239
EU Commodity Code：30021010
商品名：CARD4 (CARD 4, Caspase Recruitment Domain 4, Caspase Recruitment Domain Containing Protein 4, Caspase Recruitment Domain Family Member 4, Caspase Recruitment Domain Protein 4, Caspase Recruitment Domain-containing Protein 4, CLR 7.1, CLR7.1, NLR Family CARD Domain Containing 1, NLRC 1, NLRC1, NOD 1, Nod1, NOD1 Protein, NOD1_HUMAN, Nucleotide Binding Oligomerization Domain Containing 1, Nucleotide Binding Oligomerization Domain Leucine Rich Repeat and CARD Domain Containing 1, Nucleotide-binding Oligomerization Domain-containing Protein 1, Protein Nod1) 100ug
