Calretinin, also known as 29kD calbindin, is a vitamin D-dependent calcium-binding protein involved in calcium signaling.
In humans, the calretinin protein is encoded by the CALB2 gene.
This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily.
Members of this protein family have six EF-hand domains which bind calcium.
Calretinin is mapped to 16q22.2.
This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering.
It also functions as a modulator of neuronal excitability and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human Calretinin. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa234-262, EMNIQQLTNYRKSVMSLAEAGKLYRKDLE, from human Calretinin at C-terminal, different from the related mouse and rat sequences by 1aa.
