C5a is a protein fragment released from complement component C5.
This gene is mapped to 9q33.2.
The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes.
This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge.
An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase.
The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components.
Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections.
Defects in this gene have also been linked to susceptibility to liver fibrosis and to rheumatoid arthritis.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1-0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.
Specificity：Recognizes rat C5a.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide corresponding to aa59-77, TIADKIRKESHHKGMLLGR at the C-terminal, of rat C5a. The sequence is different in mouse by five amino acids.
