84-3431-08　Bub1 (BUB1 Budding Uninhibited by Benzimidazoles 1 Homolog, BUB1 Budding Uninhibited by Benzimidazoles 1 Homolog (Yeast), BUB1 Mitotic Checkpoint Serine/Threonine Kinase, BUB1, S. cerevisiae, Homolog of, BUB1_HUMAN, BUB1A, BUB1L, Budding Uninhibited by Benzimidazoles 1 (Yeast Homolog), Budding Uninhibited by Benzimidazoles 1 Homolog, Budding Uninhibited by Benzimidazoles 1, S. cerevisiae, Homo ...　303159

特徴

• BUB1, also known as mitotic checkpoint serine/threonine kinase, is an enzyme that in humans is encoded by the BUB1 gene.
• It is mapped to 2q13.
• BUB1 is first identified in genetic screens of Saccharomyces cerevisiae.
• The protein is bound to kinetochores and plays a key role in the establishment of the mitotic spindle checkpoint and chromosome congression.
• The mitotic checkpoint kinase is evolutionary conserved in organisms as diverse as Saccharomyces cerevisiae and humans.
• Loss-of-function mutations or absence of BUB1 has been reported to result in aneuploidy, chromosomal instability (CIN) and premature senescence.
• The protein kinase BUB1 possesses versatile and distinct functions during the cell cycle, mainly in the SAC and chromosome alignment during metaphase.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.