84-3431-06　BRCA1 (BRAC 1, BRCA 1, BRCA1/BRCA2 Containing Complex Subunit 1, BRCA1/BRCA2-containing Complex, Subunit 1, BRCA1_HUMAN, BRCAI, BRCC 1, BRCC1, Breast and Ovarian Cancer Susceptibility Protein 1, Breast Cancer 1, Breast Cancer 1 Early Onset, Breast Cancer Type 1 Susceptibility Protein, Breast Ovarian Cancer Susceptibility, Breast Ovarian Cancer Susceptibility, BROVCA1, IRIS, Papillary Serous Ca ...　303156

特徴

• BRCA1, also known as BRCC1, is a gene which mapping to 17q21.3.
• This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor.
• The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC).
• BRCA1 product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes.
• This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination.
• In addition to it, BRCA1 may normally serve as a negative regulator of mammary epithelial cell growth and that this function is compromised in breast cancer either by direct mutation or by alterations in gene expression.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.