Autophagy protein 5 is a protein that in humans is encoded by the ATG5 gene.
It is also known as APG5 or ASP, and this gene is mapped to 6q21.
It is found that knockdown of ATG5 in hepatocytes increased triglyceride levels with oleate or a second endogenous stimulus for triglyceride formation.
These hepatocytes with ATG5 knockdown also had increased lipid droplet number and size.
ATG5 is an E3 ubiquitin ligase which is necessary for autophagy due to its role in autophagosome elongation.
It is activated by ATG7 and forms a complex with ATG12 and ATG16L1.
This complex is necessary for LC3-1 conjugation to PE to form LC3-II.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human APG5L. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa82-97, DRFDQFWAINRKLMEY, from the middle region of human APG5L, identical to the related mouse sequence, and different from the related rat sequence by 1aa.
