Autophagy-related protein 7 is a protein that in humans is encoded by the ATG7 gene.
It is mapped to 3p25.3.
This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7.
In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes.
The protein also shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes.
ATG7 is essential for the Apg12 conjugation system that mediates membrane fusion in autophagy.
It is found that when nutrients are limited, ATG7 can regulate p53-dependent cell cycle and cell death pathways.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human Apg7. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa652-669, KVLDQYEREGFNFLAKVF, from human Apg7 at C-terminus, different from the related mouse sequence by 1aa, and from the related rat sequence by 2aa.
Shelf Life：1year
Swiss Prot Number：O95352
EU Commodity Code：30021010
商品名：Apg7, CT (Apg 7, APG7 Autophagy 7 Like, APG7 Autophagy 7-like (S. cerevisiae), APG7 Like, APG7, S. cerevisiae, Homolog of, APG7-like, APG7L, ATG 7, ATG12-activating Enzyme E1 ATG7, Atg7, ATG7 Autophagy Related 7 Homolog (S. cerevisiae), ATG7 Autophagy Related 7 Homolog, Atg7l, Autophagy 7, S. cerevisiae, Homolog of, Autophagy Related Protein 7, Autophagy-related 7 (Yeast), Autophagy-related Protein 7, GSA 7, GSA7, HAGP7, Ubiquitin Activating Enzyme E1 Like Protein, Ubiquitin-activating Enzyme E1-like Protein, Ubiquitin-like Modifier-activating Enzyme ATG7) 100ug
