APEX1, also called apurinic endonuclease (APE), is a DNA repair enzyme having apurinic/apyrimidinic (AP) endonuclease, 3-prime, 5-prime-exonuclease, DNA 3-prime repair diesterase, and DNA 3-prime-phosphatase activities.
The human APEX1 gene consists of 5 exons spanning 2.64 kb and exists as a single copy in the haploid genome.
Using in situ hybridization, the APEX1 gene is mapped to 14q11.2-q12.
The predicted APEX1 protein, which contained probable nuclear transport signals, was identified as a member of a family of DNA repair enzymes found in lower organisms.
The abundance of the large form of APEX1 was increased in leiomyoma extracts relative to myometrial tissue extracts, and the large form was dominant in cell lines derived from leiomyosarcomas.
The exonuclease activity of nuclear APEX1 can remove the anti-HIV nucleoside analogs AZT and D4T from the 3-prime terminus of a nick more efficiently than can cytosolic exonucleases.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions
Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human APE. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human APE expressed in E. coli. (Position: P2-L318). Species sequence homology: mouse (94%) and rat (93%)
Shelf Life：1year
Swiss Prot Number：Q5TZP7
EU Commodity Code：30021010
商品名：APE (AP Endonuclease 1, AP Endonuclease Class I, AP Lyase, APE 1, APE1, APE-1, APEN, APEX 1, APEX, APEX Nuclease (Multifunctional DNA Repair Enzyme) 1, Apex Nuclease 1, Apex Nuclease, APEX1, APEX1_HUMAN, Apurinic Endonuclease, Apurinic-apyrimidinic Endonuclease 1, Apurinic/Apyrimidinic (Abasic) Endonuclease, Apurinic/Apyrimidinic Endonuclease 1, Apurinic/Apyrimidinic Exonuclease, APX, BAP1, Deoxyribonuclease (Apurinic or Apyrimidinic), DNA (Apurinic or Apyrimidinic Site) Lyase, DNA-(Apurinic or Apyrimidinic Site) Lyase, Mitochondrial, HAP 1, HAP1, Human Apurinic Endonuclease 1, Multifunctional DNA Repair Enzyme, Redox Factor 1, Redox Factor-1, REF 1, REF 1 Protein, REF-1, REF1, REF1 Protein) 100ug
