Anoctamin-1 (ANO1), also known as oral cancer overexpressed 2(ORAOV2) or tumor-amplified and overexpressed sequence 2 (TMEM16A), is a protein that in humans is encoded by the ANO1 gene.
This gene belongs to a family of membrane proteins containing 8 transmembrane segments, and it is mapped to 11q13.3.
ANO1 is a candidate calcium-activated chloride channel that mediates receptor-activated chloride currents in diverse physiologic processes, and it is though to be responsible for a voltage-sensitive calcium-activated chloride current.
Its overexpression was reported in esophageal squamous cell carcinoma and breast cancer progression Crofelemer, an antidiarrhoeal, inhibits this channel.
ANO1 has eight transmembrane domains, its pore is large and non-selective, allowing other negatively charged species to permeate.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human TMEM16A. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa927-943, KVLMVELFMREEQDKQQ, from human TMEM16A at C-terminus, different from the related mouse and rat sequences by 1aa.
Shelf Life：1year
Swiss Prot Number：Q5XXA6
EU Commodity Code：30021010
商品名：ANO1, CT (TMEM16A, ANO 1, ANO1_HUMAN, Anoctamin 1, Anoctamin 1 Calcium Activated Chloride Channel, Anoctamin-1, Anoctamin1, Discovered on Gastrointestinal Stromal Tumors Protein 1, DOG 1, Membrane Protein, Oral Cancer Overexpressed 2, Oral Cancer Overexpressed Protein 2, ORAOV 2, ORAOV2, TAOS 2, TAOS2, TMEM 16A, Transmembrane Protein 16A (Eight Membrane Spanning Domains), Transmembrane Protein 16A, Tumor Amplified and Overexpressed Sequence 2, Tumor-amplified and Overexpressed Sequence 2) 100ug
