Arachidonate 15-lipoxygenase, also known as 15 LOX or LOG15, is an enzyme that in humans is encoded by the ALOX15 gene.
This gene is mapped to 17p13.2.
It is found that ALOX15 is a mutator gene.
ALOX15 gene product is implicated in anti-inflammation, membrane remodeling, and cancer development/metastasis.
Loss of the TP53 gene, or gain-of-function activities resulting from the expression of its mutant forms regulates ALOX15 promoter activity in human and in mouse, albeit in directionally opposite manners.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions
Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human ALOX15.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human ALOX15 expressed in E.coli. (Position: G2-P337). Species sequence homology: mouse (72%) and rat (73%)
