AKT2 is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains.
This gene is mapped to 19q13.2.
AKT2 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis.
AKT2 seems also to be the principal isoform responsible of the regulation of glucose uptake.
AKT2 is also specifically involved in skeletal muscle differentiation, one of its substrates in this process being ANKRD2.
Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions
Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
Immunohistochemistry (frozen): 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human AKT2. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa454-481, DRYDSLGLLELDQRTHFPQFSYSASIRE, from human AKT2 at C-terminus.
Shelf Life：1year
Swiss Prot Number：P31751
EU Commodity Code：30021010
商品名：AKT2, CT (AKT 2, Akt2, AKT2 Protein, AKT2_HUMAN, HIHGHH, Murine Thymoma Viral (V akt) Homolog 2, Murine Thymoma Viral (V-akt) Homolog-2, PKB, PKB beta, PKBB, PKBbeta, PRKBB, Protein Kinase Akt 2, Protein Kinase Akt-2, Protein Kinase B beta, RAC beta, RAC beta Serine Threonine Protein Kinase, RAC PK beta, Rac Protein Kinase beta, RAC-Beta, RAC-beta Serine/Threonine-protein Kinase, RAC-PK-beta, RACbeta, V Akt Murine Thymoma Viral Oncogene Homolog 2) 100ug
