Apoptosis-inducing factor 1, mitochondrial, also known as AIF or PDCD8 is a protein that in humans is encoded by the AIFM1 gene.
AIFM1 gene is mapped to Xq26.1 based on an alignment of the AIFM1 sequence with the genomic sequence.
This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells.
Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation.
In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9.
Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy.
A related pseudogene has been identified on chromosome 10.
Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
Immunohistochemistry (frozen): 0.5-1ug/mlImmunocytochemistry: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human AIFM1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa596-613, EQHEDLNEVAKLFNIHED, from human AIFM1 at C-terminus, identical to the related rat and mouse sequences.
Shelf Life：1year
Swiss Prot Number：O95831
EU Commodity Code：30021010
商品名：AIFM1, CT (AIF, Apoptosis Inducing Factor 1, Mitochondrial, Apoptosis Inducing Factor, Apoptosis Inducing Factor, Mitochondrion Associated 1, Apoptosis-inducing Factor 1, CMTX4, COXPD6, Harlequin, Hq, mAIF, Mitochondrial, PDCD 8, PDCD8, Programmed Cell Death 8 (Apoptosis Inducing Factor), Programmed Cell Death 8, Programmed Cell Death 8 Isoform 1, Programmed Cell Death 8 Isoform 2, Programmed Cell Death 8 Isoform 3, Programmed Cell Death 8 Isoform 5, Programmed Cell Death Protein 8, Programmed Cell Death Protein 8 Mitochondrial, Programmed Cell Death Protein 8 Mitochondrial Precursor, Striatal Apoptosis Inducing Factor) 100ug
