ADIPOQ (Adipocyte-, C1q-, and Collagen Domain-Containing), also known as APM1, ADPN or ACDC, is a protein which in humans is encoded by the ADIPOQ gene.
Using FISH, Das et al.
(2001) mapped the mouse Acrp30 gene to chromosome 16 in a region showing homology of synteny with human 3q27.
By RNase protection and Western blot analysis, Schaffler et al.
(1999) showed that APM1 is expressed by differentiated adipocytes as a 33kD protein that is also detectable in serum.
By sequence comparisons, they found links between APM1 and TNF family ligands as well as to cytokines expressed by T cells.
Adiponectin is a protein hormone that modulates a number of metabolic processes, including glucose regulation and fatty acid oxidation.
Adiponectin is exclusively secreted from adipose tissue (and also from the placenta in pregnancy) into the bloodstream and is very abundant in plasma relative to many hormones.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~1ng/lane under reducing conditions
Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human Adiponectin.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human Adiponectin expressed in E. coli. (Position: E19-N244). Species sequence homology: mouse (85%)
Shelf Life：1year
Swiss Prot Number：Q15848
EU Commodity Code：30021010
商品名：Adiponectin (30kD Adipocyte Complement Related Protein, 30kD Adipocyte Complement-related Protein, ACDC, ACRP 30, ACRP30, ADIPO_HUMAN, Adipocyte, Adipocyte C1q and Collagen Domain Containing Protein, Adipocyte Complement Related 30kD Protein, Adipocyte Complement Related Protein of 30kD, Adipocyte Complement-related 30kD Protein, Adipocyte-specific Secretory Protein, Adiponectin Precursor, Adiponectin, C1Q and Collagen Domain Containing, AdipoQ, Adipose Most Abundant Gene Transcript 1, Adipose Most Abundant Gene Transcript 1 Protein, Adipose Specific Collagen Like Factor, ADIPQTL1, ADPN, APM 1, ApM-1, ApM1, C1q and Collagen Domain-containing Protein, GBP 28, GBP28, Gelatin Binding Protein, Gelatin Binding Protein 28, Gelatin-binding Protein) 100ug
