ABI1 is a human homolog of mouse Abl-interactor-1 (Abi1), mapped on 10p11.2.
ABI1 participates in the transduction of signals from Ras to Rac by regulating Rac-specific guanine nucleotide exchange factor (GEF) activities.
It dramatically promoted ABL1-mediated tyrosine phosphorylation of MENA, but not of other substrates.
Abi-1 regulates c-Abl-mediated phosphorylation of Mena by interacting with both proteins.
ABI1 plays a role in the leukemogenesis by translocating to MLL.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.1ng/lane under reducing conditions
Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human Abi-1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa151-175, HGVKWLKAKHGNNQPARTGTLSRTN, from human Abi-1 at N-terminus, identical to the related mouse and rat sequences.
Shelf Life：1year
Swiss Prot Number：Q8IZP0
EU Commodity Code：30021010
商品名：Abi-1, NT (SSH3BP1, Abelson Interactor 1, ABI 1, ABI1, ABI1_HUMAN, Abl Binding Protein 4, Abl Interactor 1, Abl Interactor Protein 1, Abl Interactor Protein 1 Long, Abl-binding Protein 4, AblBP4, Ablphilin 1, E3B1, Eps8 Binding Protein, Eps8 SH3 Domain Binding Protein, Eps8 SH3 Domain-binding Protein, Eps8-binding Protein, Hssh3bp1, Interactor Protein AblBP4, NAP1, Nap1 Binding Protein, Nap1-binding Protein, NAP1BP, Spectrin SH3 Domain Binding Protein 1, Spectrin SH3 Domain-binding Protein 1, SSH3BP) 100ug
