84-3430-44　ABCC4 (MRP4, ABCC 4, ATP Binding Cassette Sub Family C (CFTR/MRP) Member 4, ATP Binding Cassette Sub Family C Member 4, ATP-binding Cassette Sub-family C Member 4, Canalicular Multispecific Organic Anion Transporter ABC Superfamily, Canalicular Multispecific Organic Anion Transporter, MOAT B, MOAT-B, MOATB, MRP 4, MRP/cMOAT Related ABC Transporter, MRP/cMOAT-related ABC Transporter, Multi Spec ...　303088

特徴

• ABCC4 (Atp-binding cassette, subfamily c, member 4) also known as MRP4 or MOATB, is a protein that in humans is encoded by the ABCC4 gene.
• It belongs to a large family of transmembrane proteins involved in active transport of substrates out of cells.
• This gene is mapped to chromosome 13q32.
• ABCC4 acts as an independent regulator of intracellular cyclic nucleotide levels and as a mediator of cAMP-dependent signal transduction to the nucleus.
• The antiproliferative effect of ABCC4 inhibition was related to cAMP-dependent PKA activation and CREB phosphorylation.
• Pharmacologic inhibition of ABCC4 activity or knockdown of ABCC4 via RNA interference resulted in reduced migration of DCs from human skin explants and of in vitro-generated Langerhans cells.
• It has been found that ABCC4 contributes to migration of DCs toward draining lymph nodes and therefore has a role in the initiation of an immune response.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions
• Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.