Urocortin 2 (Ucn 2), also known as stresscopin-related peptide, is a novel predicted neuropeptide related to corticotropin-releasing factor (CRF).
The peptide consisting of 38 amino acid residues was first demonstrated to be expressed centrally and to bind selectively to type 2 CRF receptor (CRFR2).
In the rodent, Ucn 2 transcripts were shown to be expressed in the discrete regions of the central nervous system including stress-related cell groups in the hypothalamus and brainstem.
More recently, the expression of Ucn 2 transcripts was detected in the olfactory bulb, pituitary, cortex, hypothalamus, and spinal cord.
Ucn 2 mRNA was also found to be expressed widely in a variety of peripheral tissues, most highly in the skin and skeletal muscle tissues.
Ucn 2-like immunoreactivity was detected by RIA in acid extracts of mouse brain, muscle, and skin.
Immunohistochemically Ucn 2 was found in both skin epidermis and adnexal structures and in the skeletal muscle myocytes.
Applications:Suitable for use in Direct ELISA, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Direct ELISA: 1:3200Immunohistochemistry: 1:500Optimal dilutions to be determined by the researcher.
